心臟成纖維細(xì)胞為心肌細(xì)胞提供結(jié)構(gòu)支持，并負(fù)責(zé)在生長(zhǎng)和病理生理?xiàng)l件下心肌細(xì)胞外基質(zhì)的合成。CF是心肌損傷反應(yīng)的重要細(xì)胞成分和旁分泌生長(zhǎng)因子的來源。CF的增殖和基質(zhì)合成對(duì)于心肌梗塞、心肌纖維化部位瘢痕形成具有重要作用。CF的培養(yǎng)已被廣泛應(yīng)用為生理（運(yùn)動(dòng)）和病理（高血壓）應(yīng)激下心臟基質(zhì)重塑研究的模型。CF的培養(yǎng)已被廣泛應(yīng)用為生理（運(yùn)動(dòng)）和病理（高血壓）應(yīng)激下心臟基質(zhì)重塑研究的模型。CF通過間隙連接施加電緊張，影響心肌細(xì)胞的電耦合性形式。這些電耦合被認(rèn)為在心房復(fù)雜的分流電描位點(diǎn)發(fā)生中起重要作用，因此是研究房顫的一個(gè)**模型。
Sciencell研究實(shí)驗(yàn)室的HCF-aa是從人胎兒心房分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度超過5×10^5/ml。細(xì)胞特性經(jīng)纖維粘連蛋白免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基 (HM, Cat. #2301)
[1] Sabri A, Short J, Guo J, Steinberg SF. (2002) ?Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes.? Circ Res. 91: 532-9.
[2] Akiyama-Uchida Y. (2002) ?Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts.? Hypertension. 40: 148-54.
[3] Burgess ML, Terracio L, Hirozane T, Borg TK. (2002) ?Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts.? Cardiovasc Pathol. 11: 78-87.
[4] Ashihara T, Haraguchi R, Nakazawa K, Namba T, Ikeda T, Nakazawa Y, Ozawa T, Ito M, Horie M, Trayanova NA. (2012) ?The role of fibroblasts in complex fractionated electrograms during persistent/permanent atrial fibrillation: implications for electrogram-based catheter ablation.? Circ Res. 110: 275-84.
